Determination of Total Vitamin C in Sugar Product with 2, 4-Dinitrophenylhydrazine

Determination of Vitamin C (Ascorbic Acid) in Orange Juices Product

This research describes a voltammetric approach to determine amounts of vitamin C (Ascorbic acid) in orange juice sample, using three screen printed electrode. The anodic currents of vitamin C were proportional to vitamin C concentration in the range of 0 – 10.0 mM with the limit of detection of 1.36 mM. The method was successfully employed with 2 μL of the working solution dropped on the elect...

Determination of Formaldehyde in Human Tissue through Derivatization with 2 , 4 - Dinitrophenylhydrazine by Square Wave Polarography Method

The polarographic behaviour of formaldehyde (FA) was studied using cyclic and square wave polarography (SWP) methods. FA was derivatized with 2.4-dinitrophenylhydrazine (DNPH). The influence of several variables (including nature of the buffer, pH, concentration, scan rate, drop size, etc.) was examined in SWP method for FA. The best SWP responses were obtained in acetate buffer pH 5.0. The pea...

Determination of vitamin C in iranian citrus fruits

A simplified 2, 4-dinitrophenylhydrazine assay for Flavonoids and its comparison with a Standard flavonoid assay

A simplified version of the 2, 4-DNPH assay for flavonoids has been improvised. The assay includes incubating test sample with DNPH in a 4.5 mL reaction volume in hot water at about 80 C containing 2 mL methanol at about 0.2 N HCl, cooling samples to room temperature, adding 3 mL 1M NaOH, and monitoring red color at 470 nm at 10-15 minutes. The reagent has been found capable of assaying both fl...

Determination of total vitamin C in fruit juices and related products by liquid chromatography: interlaboratory study.

A interlaboratory study was conducted to evaluate a liquid chromatographic (LC) procedure for the determination of total vitamin C in foods at levels of 5-60 mg/100 g. Emphasis was placed on fruit juices, although selected foods were also included in the study. Following dissolution of sample in water, endogenous dehydroascorbic acid was converted to ascorbic acid by precolumn reduction with di...